Transcriptomics aids in uncovering the metabolic shifts and molecular equipment of Schizochytrium limacinum all through biotransformation of hydrophobic substrates to docosahexaenoic acid Iqra Mariam
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The reverse genetic tactic along with a tracing experiment applying steady isotope-labeled fatty acids revealed the ELO/DES pathway is the only DHA synthesis pathway in Parietichytrium sp. Disruption with the C20 fatty acid ELO (C20ELO) and ∆4 fatty pgufa24 acid DES (∆4DES) genes with expression of ωthree fatty acid DES in this thraustochytrid permitted the manufacture of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the very best level between acknowledged microbial resources making use of fed-batch society.
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We report here that Parietichytrium sp. does not have PUFA-S and synthesizes DHA exclusively through the ELO/DES pathway. To the ideal of our information, Parietichytrium sp. is the first example of a thraustochytrid that synthesizes a large amount of DHA through a system besides the PUFA-S pathway.